Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228.

نویسندگان

  • Hikmate Abriouel
  • Anette Herrmann
  • Joachim Stärke
  • Nuha M K Yousif
  • Agus Wijaya
  • Bernhard Tauscher
  • Wilhelm Holzapfel
  • Charles M A P Franz
چکیده

Lactobacillus plantarum CNRZ 1228 exhibited heme-dependent catalase activity under environmental conditions similar to those encountered during sausage fermentation. The 1,455-bp catalase gene (katL) was cloned and encoded a protein of 484 amino acids. Expression of katL in a heterologous host showed that katL encodes a functional catalase. PCR screening of selected strains of lactic acid bacteria for katL indicated the presence of similar genes in other strains of lactobacilli.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The S-layer gene of Lactobacillus helveticus CNRZ 892: cloning, sequence and heterologous expression.

Lactobacillus helveticus CNRZ 892 contains a surface layer (S-layer) composed of protein monomers of 43 kDa organized in regular arrays. The gene encoding this protein (slpH) has been cloned in Escherichia coli and sequenced. slpH consists of 440 codons and is preceded by a ribosome-binding site (RBS) and followed by a putative rho-independent terminator. Indeed, Northern analysis revealed that...

متن کامل

Distribution and Characteristics of the Catalases of Lactobacillaceae.

Johnston, M. A. (Cornell University, Ithaca, N.Y.), and E. A. Delwiche. Distribution and characteristics of the catalases of Lactobacillaceae. J. Bacteriol. 90:347-351. 1965.-Certain strains of lactobacilli and pediococci incorporated hematin during growth, with the concomitant formation of cyanide- and azide-sensitive catalase. Three of five strains of lactobacilli and five of 25 strains of pe...

متن کامل

Cloning strategies for heterologous expression of the bacteriocin enterocin A by Lactobacillussakei Lb790, Lb. plantarum NC8 and Lb. casei CECT475

BACKGROUND Bacteriocins produced by lactic acid bacteria (LAB) attract considerable interest as natural and nontoxic food preservatives and as therapeutics whereas the bacteriocin-producing LAB are considered potential probiotics for food, human and veterinary applications, and in the animal production field. Within LAB the lactobacilli are increasingly used as starter cultures for food preserv...

متن کامل

Adaptation of the nisin-controlled expression system in Lactobacillus plantarum: a tool to study in vivo biological effects.

The potential of lactic acid bacteria as live vehicles for the production and delivery of therapeutic molecules is being actively investigated today. For future applications it is essential to be able to establish dose-response curves for the targeted biological effect and thus to control the production of a heterologous biopeptide by a live lactobacillus. We therefore implemented in Lactobacil...

متن کامل

Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum

BACKGROUND Two sets of overlapping genes, lacLMReu and lacLMAci, encoding heterodimeric β-galactosidases from Lactobacillus reuteri and Lactobacillus acidophilus, respectively, have previously been cloned and expressed using the pSIP vector system and Lactobacillus plantarum WCSF1 as host. Despite the high similarity between these lacLM genes and the use of identical cloning and expression stra...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 70 1  شماره 

صفحات  -

تاریخ انتشار 2004